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Cellosaurus publication CLPUB00288

Publication number CLPUB00288
Authors Caputo J.L., Hay R.J., Williams C.D.
Title The isolation and properties of an epithelial cell strain from rhesus monkey bronchus.
Citation In Vitro 15:222-223(1979)
Abstract An epithelial cell strain dependent upon epidermal growth factor (EGF) for proliferation has been isolated from explant cultures of monkey bronchi. Segments of approximately 3 mm2 were placed in 60 mm plates containing glass chips. The standard growth medium used was F12K with 15% FBS. Chips supporting epithelial outgrowths apparently free of fibroblasts were transferred to 16 mm wells of cluster plates. Cells thus isolated required both EGF (10-30 ng/ml) and the presence of irradiated lung fibroblasts (ATCC CCL-171 - MRC5) for subsequent propagation to mass population densities. Epithelial cultures could be propagated serially for more than 6 months with over 33 population doublings. The omission of EGF at any point resulted in an obvious decline in proliferation rates and the appearance of vacuolated cells. Biochemical studies showed that addition of EGF resulted in a 20-fold increase in 3H-thymidine incorporation. Dexamethasone (10^-8 M) acted synergistically with EGF to double 3H-thymidine uptake. Histochemical staining showed that vigorous cultures contained abundant PAS-positive cytoplasmic droplets. The cells also exhibited high levels of malic dehydrogenase as did epithelia lining bronchial lumina. Examination of the fine structure of cultured bronchial cells revealed desmosomes plus a variety of electronlucent droplets and dense membrane-bound bodies with somewhat heterogeneous matrices. The cell strain has been preserved in liquid nitrogen and can be recovered readily. Further characterizations are in progress.
Cell lines CVCL_4492; 4MBr-5