Cellosaurus publication CLPUB00295

Publication number CLPUB00295
Authors Yokoi T., Homma H., Odajima T.
Title Establishment and characterization of OSC-19 cell line in serum- and protein-free culture.
Citation Tumor Res. 24:57-73(1989)
Web pages http://ci.nii.ac.jp/naid/120001796460/en
Abstract Morphological properties and keratin profiles of a human squamous cell carcinoma cell line, OSC-19 in chemically defined media were investigated and compared to those under conditions in which 10% fetal bovine serum was supplemented. OSC-19 cells were adapted to continuous culture in serum-free (SF) maintained in SF medium were further adapted to protein-free medium with no exogenous factors. The cells cultivated in SF medium had a tendency to elongate and grew in scrollwork-like pattern, while the cells under serum containing condition were polygonal in shape and grew in cobblestone pattern. In SF culture condition, the cells formed focal stratifications and an apparent electron microscopic examination. With the morphological changes, keratin profiles were also changed. The cells cultured in SF medium produced two new major keratins (50 and 56 kd) in addition to five major keratins that were present in cells cultured in serum supplemented medium.
Cell lines CVCL_3086; OSC-19