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Cellosaurus publication CLPUB00296

Publication number CLPUB00296
Authors Yokoi T., Yamaguchi A., Tetsuyo O., Kazunori F.
Title Establishment and characterization of a human cell line derived from a squamous cell carcinoma of the tongue.
Citation Tumor Res. 23:43-57(1989)
Web pages https://eurekamag.com/research/007/312/007312593.php
Abstract A cell line designated as OSC-19 was established from the metastatic tumor which was found in a cervical lymph node of a male patient suffering squamous cell carcinoma (SCC) of tongue. OSC-19 cells were polygonal in shape and grew in a cobblestone pattern. Desmosomes and microvilli were observed by electron microscopic examination, but tonofilament bundles were scarce. However immunofluorescence studies showed cytokeratins in the cytoplasms of OSC-19 cells. When inoculated into nude mice, OSC-19 cells had many distinctive desmosomes and plenty of tonofilament bundles. These observations suggested strongly that OSC-19 cell line was SCC in origin. OSC-19 cells had receptors of epidermal growth factor (EGF), but the growth of the cells in dish culture was inhibited by 1 to 100 ng/ml EGF in dose-dependent manners. OSC-19 cells could not grow in soft agar, suggesting that they were strongly anchorage dependent. However, in contrast to dish culture, EGF stimulated colony formation of OSC-19 cells in soft agar. These results suggested that EGF has complex effects on the cell growth. Cells may not be grown when cultured directly in the serum free medium after thawing the frozen ampule. First culture from the recovery is recommended to use medium containing serum.
Cell lines CVCL_3086; OSC-19