| Publication number |
CLPUB00310 |
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| Authors |
Rajabalian S., Bahrami Z.S., Farahat V., Shokri F. |
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| Title |
Supportive effects of human embryonic fibroblast cell lines on growth and proliferation of EBV-transformed lymphoblastoid cells. |
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| Citation |
Iran. Biomed. J. 7:147-153(2003) |
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| Web pages |
http://ibj.pasteur.ac.ir/article-1-521-en.html |
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| Abstract |
Human diploid fibroblast cells produce a spectrum of necessary growth
factors and extracellular matrix (ECM) components essential for growth and
proliferation of a variety of other cell types. In this study, the effect
of five human embryonic fibroblast cell lines, isolated from liver, lung,
skin and foreskin tissues, was investigated. A coculture system analyse
was employed to cloning efficiency (CE) and DNA synthesis of a human
Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) in
long-and short-term cultures. The fibroblast cells were used as feeder
layer after treatment with mitomycin C. Optimal density of the feeder
cells induced 10 to 43 times higher CE than cultures supplemented with
conditioned media (CM) or cultures without a feeder layer. The stimulatory
effect of the feeder cells was partly associated to their tissue origin,
with the lung and liver fibroblasts being the most and least effective
feeder cells, respectively. Short-term cultures of LCL cells with feeder
cells or their CM resulted in a marginal increase in DNA synthesis and
proliferation as evidenced by the index of 3H-thymidine incorporation. Our
results demonstrated supportive effects of feeder cells on the LCL growth,
which can not be replaced by their CM. These supportive effects were
partly associated with cell density and tissue origin of the feeder cells.
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| Cell lines |
CVCL_9V94; HFFF-PI 6 CVCL_9V33; HFLF-PI 4 CVCL_9V95; HFLF-PI 5 CVCL_9V96; HFSF-PI 3 CVCL_9V48; LCL-PI 4 |
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