||In developing techniques and media for the isolation and cultivation of
cell lines from human solid tumors, two recent isolates are of interest
for their unusual characteristics. Both were isolated by the spinner
spillout technique and grown in medium L-15 plus various additives. SW13,
epithelial-like cells obtained from a small cell carcinoma of the adrenal
cortex, grew in colonies and domed. Ultrastructurally, they contained
numerous bulb-type gap junctions. By the 25th passage, the cells lost most
of their ability to dome, and significantly fewer gap junctions were noted.
SW48 was derived from an adenocarcinoma of the colon. The cells grew as
grape-like clusters their first 7 months in vitro and then partially as
monolayers of epithelial-like cells. In platform suspension cultures, they
grew as multicell spheroids which enlarge and develop necrotic centers.
Ultrastructurally they were epithelial-like cells tightly enmeshed by
their microvilli and joined by desmosomes. The multicell spheroids formed
a tight layer of cells around their periphery and a similar layer to wall
off the necrotic area. The latter layer of cells disappeared when the area
of necrosis became extensive. This cell line is an excellent in vitro
model of human nodular carcinomas. No reference could be found in the
literature of similar cell lines obtained from human tissues.