| Abstract |
A majority of human lymphoid cell lines have B cell characteristics: they
produce immunoglobulin and lack the ability to form rosettes with sheep
red blood cells. Without exception, the 272 cell lines derived from normal
persons have B cell characteristics. B cell characteristics predominate in
abnormal cell lines derived from Burkitt's lymphoma and chronic
myclogenous leukemia. A cell line derived from multiple myeloma produces
only light chains and does not, form rosettes. Several cell lines
established from patients with lymphatic leukemia have either T cell
characteristics or a mixture of T and B cell characteristics. Cell lines
8402, 8412, 8422, 8432, 8442, 8452, 8382, and 8392 from the same
individual demonstrate that abnormal lymphoid cell lines may vary in their
reactions. Early in culture these lines carried the chromosome markers
present in the donor. Several cell lines are distinguished by their lack
of clumping in stationary cultures; these are most like T cells. After 4
months in culture, several lines with T cell characteristics began to form
clumps and simultaneously lost their ability to form rosettes with sheep
red blood cells. These cells that form clumps produce detectable amounts
of immunoglobulin and contain a more normal chromosome constitution.
Ultramorphological measurements indicate that the T cells contain fewer
mitochondria, less rough endoplasmic reticulum, and more lysosomes; are
smaller than B cells; and probably represent the leukemic lymphocytes.
|
|---|