Cellosaurus publication CLPUB00338

Publication number CLPUB00338
Authors Moore G.E., Woods L.K., Minowada J., Mitchen J.R.
Title Establishment of leukemia cell line with T cell characteristics.
Citation In Vitro 8:434-434(1973)
Abstract A majority of human lymphoid cell lines have B cell characteristics: they produce immunoglobulin and lack the ability to form rosettes with sheep red blood cells. Without exception, the 272 cell lines derived from normal persons have B cell characteristics. B cell characteristics predominate in abnormal cell lines derived from Burkitt's lymphoma and chronic myclogenous leukemia. A cell line derived from multiple myeloma produces only light chains and does not, form rosettes. Several cell lines established from patients with lymphatic leukemia have either T cell characteristics or a mixture of T and B cell characteristics. Cell lines 8402, 8412, 8422, 8432, 8442, 8452, 8382, and 8392 from the same individual demonstrate that abnormal lymphoid cell lines may vary in their reactions. Early in culture these lines carried the chromosome markers present in the donor. Several cell lines are distinguished by their lack of clumping in stationary cultures; these are most like T cells. After 4 months in culture, several lines with T cell characteristics began to form clumps and simultaneously lost their ability to form rosettes with sheep red blood cells. These cells that form clumps produce detectable amounts of immunoglobulin and contain a more normal chromosome constitution. Ultramorphological measurements indicate that the T cells contain fewer mitochondria, less rough endoplasmic reticulum, and more lysosomes; are smaller than B cells; and probably represent the leukemic lymphocytes.
Cell lines CVCL_1667; RPMI-8402