| Publication number |
CLPUB00354 |
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| Authors |
Xiang M., Lu S., Gao Q.-H., Huang H.-J., Chen Z.-H., Tao B.-F., Zhan C.-Y., Qian Y.-G., Sun R.-L., Xia Y., Tong W.-W., Wang L.-F., Hua J., Tao L.-W. |
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| Title |
A new cell line from goat embryonic kidney may be a candidate to produce influenza virus vaccine. |
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| Citation |
Indian J. Anim. Sci. 85:828-831(2015) |
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| Web pages |
https://www.researchgate.net/publication/282951008 |
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| Abstract |
This study established a goat embryonic kidney (GEK) cell line which was
susceptible to influenza virus. Kidney cells were separated from fetus of
Matou goat and GEK cell line was established by cell biology techniques.
Tumorigenicity of GEK cell line was tested by tumorigenesis assay, and its
marker protein expression was tested by immunohistochemistry. Receptors of
influenza virus and susceptibility to H1N1 influenza virus were also
tested by immunofluorescence method and cell infection assay, respectively.
Results showed that GEK cells transplanted into BALB/c nude mice could not
induce tumorigenesis. Immunohistochemical analysis showed that the cells
expressed cytokeratin 18 (CK-18) and vimentin which are marker proteins of
renal epithelial cells. Immunofluorescent assay indicated that the
abundance of alpha2,3-sialic acid (SA) receptor and alpha2,6-SA receptor
on the surface of GEK cells were higher than that of Madin-Darby canine
kidney (MDCK) cells. The influenza virus titers in GEK cells were similar
to that of MDCK cells. Therefore, the GEK cell line established here will
possibly contribute to the future influenza virus isolation and vaccines
development.
|
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| Cell lines |
CVCL_GU03; GEK |
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