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Cellosaurus publication CLPUB00368

Publication number CLPUB00368
Authors Polanowski F.P., Gaffney E.V., Burke R.E.
Title HBL-100, a cell line established from human breast milk.
Citation In Vitro 12:328-328(1976)
Web pages http://link.springer.com/article/10.1007/BF02801988
Abstract The purpose of this study was to characterize a line of cells established from a normal source of human mammary epithelium. Human milk samples collected from nursing mothers during the first week postpartum contain both colony forming epithelium, desquamated from ducts and alveoli; and foam cells responsible for the synthesis of milk products. These cells can be maintained in monolayer culture for several weeks. Two samples of milk from the 300 patients initially surveyed contained cells capable of continuous growth in vitro. One cell line, HBL-100, has been transferred through 90 weekly passages. These cells do not form tumors in nude mice but will grow in soft agar. Of 60 metaphases examined during passage 8 the number of chromosomes per cell varied from 58 to 73 with a hypertriploid mean of 66. The 80 of 83 metaphases scanned during passage 50 had chromosome numbers in the low 60 range. No HeLa marker chromosomes were found, and cells exhibited a B-type G6PD electrophoretic pattern. Most of the metaphases had one or more minute chromosomes. Electron micrographs revealed the presence of desmosomes. Although radioimmunoassay for alpha- lactalbumin was negative, the cells do synthesize a small amount of lactose. HBL-100 cells respond to prolactin and/or estrogen by synthesizing increased amounts of isoelectrically precipitable casein. These observations demonstrated that this cell line may classify as: (1) epithelial, originating from a normal source; (2) transformed but not malignant; and (3) mammary inferred from their capacity to respond to lactogenic hormones.
Cell lines CVCL_4362; HBL-100