| Publication number |
CLPUB00430 |
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| Authors |
Peter H.-J., Gerber H., Studer H., Groscurth P., Zakarija M. |
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| Title |
Thyroid cell lines forming follicle-like lumina in vitro. |
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| Citation |
(In) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon A., Gross J., Hennemann G. (eds.); pp.579-582; A A Balkema Publishers; Rotterdam (1991) |
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| Abstract |
The morphogenetic processes leading to the formation of thyroid follicles
in vitro have been intensely studied in a variety of cell culture systems
such as in monolayer and in collagen cultures. Two basic mechanisms of
follicle morphogenesis have been described: One involves the fusion of
originally intracellular lumina, while the other postulates the pro-
gressive enlargement of primarily intercellular spaces. Most studies were
performed on primary cultures of thyroid cells during early in vitro
passages, since follicular cells in vitro display only a limited growth
potential and rapidly dedifferentiate. Only a few cell lines have been
reported to retain the ability to form follicle-like structures during
long term in vitro culture (e.g. FRTL (but not FRTL-5), OVNIS, PORTHOS).
We here describe a continuous cell line (called PETCAT 1) prepared from
autonomously growing multinodular, hyperthyroid feline goiters, which had
a morphogenetic potential not observed in 4 other cell lines. In PETCAT
cells, the follicle forming process began within the single cell before
the first cell division. The follicle forming potential was very different
from one cell to the next. In addition, fully functional follicles were
observed after in vivo growth of a FRTL-5 mutant in nude mice.
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| Cell lines |
CVCL_0265; FRTL-5 CVCL_VG49; FRTL-5 Miami CVCL_VG33; PETCAT 1 |
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