Publication number |
CLPUB00431 |
Authors |
Inufusa H. |
Title |
Characterization and metastatic study in nude mouse of human lung adenocarcinoma cell line KUM.LK-2. |
Citation |
Kinki Daigaku Igaku Zasshi 12:631-641(1987) |
Web pages |
https://ci.nii.ac.jp/naid/110000060156/en |
Abstract |
Characterization and metastatic study were performed in new established
human lung adenocarcinoma cell line, KUM.LK-2 which produced spontaneous
lung metastasis in nude mice by subcutaneous implantation. The KUM.LK-2
cell line was established from the 2nd generation of nude mouse to which
human lung adenocarcinoma was serially transplanted. The KUM.LK-2 produced
spontaneous lung metastasis when the cells were subcutaneously implanted
into nude mice. Metastasis was not found in the cases of intra-muscle
implantation and intravenous injection. The KUM.LK-2 cell produced
carcinoembryonic antigen and carbohydrated antigen 19-9 in vitro, but
these tumor markers were not detected in the serum of KUM.LK-2
transplanted nude mice. From this study the model of spontaneous lung
metastasis of human cancer was established. It is suggested that
metastasis of human cancer in the nude mouse is not dominated only by
blood supply and new vascular genesis. And it may be concluded that the
usefulness of nude mice as an amplifier of tumor marker is bounded by
tumor line.
|
Cell lines |
CVCL_W526; KUM.LK-2 |