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Cellosaurus publication CLPUB00481

Publication number CLPUB00481
Authors Kuno H., Yoshida T.
Title Detection of human papillomavirus types 16, 18, and 33 in cell lines derived from human genital organs by polymerase chain reaction.
Citation Res. Commun. Inst. Ferment. Osaka 18:6-12(1997)
Web pages http://www.ifo.or.jp/rc_pdf/18.pdf
Abstract Human papillomavirus (HPV) types 16, 18, and 33 play a causative role in the development of uterine cervix cancer. Fourteen cell lines derived from human genital organs are maintained at the Institute for Fermentation, Osaka (IFO). To know whether these cell lines were infected by HPV types 16, 18, and 33, we tried to detect these viral DNAs by polymcrase chain reaction (PCR) with specific primers homologous to the E6 open reading frame. All seven cell lines derived from uterine cervix carcinoma possessed the HPV types 16 or 18 DNA, whereas none of seven cell lines derived from uterus body cancer or ovarian cancer possessed the HPV types 16, 18, and 33 DNA. In addition, this PCR was applicable to detect the cross-contamination of cell lines by HeLa cells.
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