||Nutritional requirements of insect cells cultured in vitro have attracted
the attention of many investigators in this field for a long time. During
the last decade many continuous cell lines were obtained. Among them were
some cell lines which had been considered very difficult to culture, if
not impossible. Success of obtaining such cell lines seems most likely to
be due to the improvement of culture media. In other words, the
modification of the culture media satisfied nutritional requirements of
the cells for multiplication. There are still many insect species from
which cell lines cannot be obtained. In order to develop culture media
which can support the cell growth of such insects, information on the
nutritional requirements of the cells will give a clue.
Unfortunately, nutritional requirements of the cultured insect cells have
not been studied extensively, and the available information is limited. In
this article, the author has tried to summarize the information on
nutritional requirements of insect cells hitherto obtained.