| Publication number |
CLPUB00514 |
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| Authors |
Cheng X.-H. |
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| Title |
Screening for differentially expressed genes in dengue infection under antibody dependent enhancement conditions. |
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| Citation |
Thesis MSc (2010), National University of Singapore, Singapore |
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| Web pages |
https://scholarbank.nus.edu.sg/handle/10635/25809 |
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| Abstract |
In this study, establishment of HL-CZ (a promonocytic cell line) as a new
platform for study of antibody dependent enhancement (ADE) of dengue virus
infection in vitro was achieved. Characteristics of HL-CZ that enable it
to support ADE were also investigated.
We performed microarray gene expression profiling to compare HL-CZ cells
infected with dengue virus under antibody dependent enhancement (ADE)
conditions versus HL-CZ cells infected to an equivalent degree but under
non-enhancing conditions. We observed differential expression of several
genes belonging to the DEAD-box family of RNA helicases (DDX). These
observations were confirmed at a protein level by immunoblotting for these
proteins in cell lysates obtained from infected cells. Subsequent
experiments employing siRNA-mediated knock-down of protein expression
suggested that DDX31 and DDX47 may be crucial in supporting infection of
dengue virus under ADE conditions.
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| Cell lines |
CVCL_WU73; HL-CZ |
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