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Cellosaurus publication CLPUB00516

Publication number CLPUB00516
Authors Lieu C.-W., Liu W.-T., Sun K.-H., Wang S.-Y.
Title Some special characteristics of the novel human promonocyte cell line, HL-CZ.
Citation J. Biomed. Lab. Sci. 5:196-204(1993)
Web pages http://readopac2.ncl.edu.tw/nclJournal/search/detail.jsp?sysId=0005397467&dtdId=000040&search_type=detail&la=ch
Abstract Some special properties of a human promonocyte cell line, HL-CZ, were characterized after long-term cloning and subcloning in vitro. About 90% of the cells was detected with CD34-like marker, and 37% of them shown CD11b positive. Meanwhile, 17% of them had phagocytosis activity when evaluated by flow cytometry. It was also found that 30-69.3% of the cells were interleukin 6 positive, 17.8-19.3% and 28-73% were interleukin 1-beta and tumor necrosis factor alpha (TNF-alpha) positive, respectively, when detected by indirect immunofluorecence. In the cytochemical studies, none of them had peroxidase or nonspecific esterase activities, whereas granular type of periodic acid Schiff reaction was seen in small population of the cells. An interesting phenomenon was that the heterogeneity was found in every subclone of long-term culture. Some huge cells were always present after 2-3 days of culture. When co-cultured with hepatitis C patient serum, the percentage of huge cells increased and an autofusion behavior was observed. This implied that some unusual mechanisms existed in this cell population.
Cell lines CVCL_WU73; HL-CZ