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Cellosaurus publication CLPUB00547

Publication number CLPUB00547
Authors Fabien N., Cespuglio R., Burlet S., Pages M.-P., Paulin C.
Title Co-localization of three neuropeptides and NADPH diaphorase in a small cell lung carcinoma cell line.
Citation Cancer J. 7:198-201(1994)
Abstract Background: The small cell lung cancer (SCLC) is an epithelial tumour with the morphological and secretory characteristics of neuroendocrine cells (neurone-specific enolase, N-CAM, neuropeptides). The establishment of a SCLC cell line provides an in vitro model to analyze the neuroendocrine secretion of these malignant cells. Methods: Bombesin, delta sleep inducing factor (DSIP) and arginine vasopressin (AVP) were studied by immunohistochemistry and by in situ hybridization. NADPH diaphorase activity was detected using an histochemical technique. Results: All the cells contained bombesin, DSIP, AVP and displayed a NADPH diaphorase activity with reflects a nitric oxide (NO) synthase activity. Conclusions: These data present the characteristics of a new SCLC cell line (CPN) and show the complexity of its neuroendocrine secretion; for example, the presence of DSIP and NO synthase activity which have not yet been demonstrated in other SCLC cell lines.
Cell lines CVCL_1146; CPC-N