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Cellosaurus publication CLPUB00549

Publication number CLPUB00549
Authors Moore J.C.
Title Establishment Of CRISPR/Cas-9 aided knockout of the ZIC2 gene in the African-American prostate cancer cell line E006AA-PR.
Citation Thesis MSc (2019), Clark Atlanta University, United States
Web pages https://hdl.handle.net/20.500.12322/cau.td:2019_moore_janelle
Abstract The largest U.S. cancer health disparity exists in prostate cancer, with African American men having the highest incidence and mortality rates. The present study evaluated the effects of ZIC2 and the underlying mechanisms in the E006 parental African-American cell line that produces tumors at accelerated growth rates because of the increase of ZIC2 genes in African- American males. We analyzed the experimental research that the overexpression of ZIC2 contributes to progression of prostate cancer. E006AA cells with overexpressed or suppressed ZIC2 were analyzed to determine phenotypic differences, PCR, cell proliferation and immunoblot assays. The expression levels of ZIC2 were analyzed by CRISPR-Cas9, Western blot and proliferation growth curves. We discovered using these experimental techniques to knockout ZIC2, reduced cell proliferation occurred. This research investigated the role of ZIC2 in prostate cancer progression and the effects of the loss or gain of function of ZIC2 by using CRISPR-Cas 9 genome editing technology.
Cell lines CVCL_4834; E006AA
CVCL_YI81; E006AA ZIC2-/- E5
CVCL_YI82; E006AA ZIC2-/- G12