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Cellosaurus publication CLPUB00550

Publication number CLPUB00550
Authors Benhamron S.
Title Validating WDR12 as a potential drug target in triple negative breast cancer.
Citation Thesis MSc (2019), University of Toronto, Canada
Web pages https://hdl.handle.net/1807/97854
Abstract Compared to other breast cancers (BC), triple negative breast cancers (TNBCs) confer lower survival and higher disease recurrence. TNBCs lack receptors targeted by current therapies, requiring new therapeutic target identification. Recently, the ribosome biogenesis protein WDR12 was identified as a potential target from RNAi databases. WDR12 belongs to the WD40-repeat family of scaffolding proteins, which have become interesting targets due to their druggable structure. I hypothesized that TNBC cell lines would be more sensitive to WDR12 knockdown (KD) due to their increased rate of ribosome biogenesis. WDR12 depletion led to decreased proliferation in all BC cell lines that could not be attributed to apoptosis or cell cycle arrest. Interestingly, WDR12 KD decreased nucleolar size to a greater extent in TNBC, indicating a potential role for WDR12 in ribosome biogenesis. In conclusion, WDR12 may be a promising therapeutic target in TNBC, although additional confirmatory testing will be required.
Cell lines CVCL_YJ25; MCF-7 shWDR12-4
CVCL_YJ26; MDA-MB-231 shWDR12-4
CVCL_YJ27; MDA-MB-468 shWDR12-4
CVCL_YJ28; SK-BR-3 shWDR12-4
CVCL_YJ29; ZR-75-1 shWDR12-4