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Cellosaurus publication CLPUB00584

Publication number CLPUB00584
Authors Motoya T., Hojo H., Suzuki T., Oboshi S.
Title Evaluation of the regrowth assay method as an in vitro drug sensitivity test and its application to cultured human gastric cancer cell lines.
Citation Acta Med. Biol. 27:49-63(1979)
Abstract There are several methods for assay of in vitro drug sensitivity of cultured cancer cells. The present study first deals with the validity of two representative methods of in vitro sensitivity tests: the growth inhibition method expressed by a sensitivity index of IC50 (50% inhibition concentration) and the regrowth assay method expressed by an index of LD90 (90% lethal dose). The regrowth assay method has been confirmed to be highly reproducible and the sensitivity index of LD90 is not influenced by the inoculation size of test cells, the day judged, and the population doubling time of test cells. Therefore, the drug sensitivity among cell lines could be evaluated by comparing their indices. On the other hand, the IC50 is not reproducible and influenced by the 3 factors above mentioned. Therefore, it might be concluded that IC50 is not suitable as an index of sensitivity of cancer cells to anticancer drugs at least in terms of cytocidal activity. Secondly, the result of sensitivity tests of 9 human stomach cancer cell lines to Mitomycin C measured by regrowth assay is reported. In general, cell lines derived from poorly differentiated adenocarcinoma are more sensitive to the test drug than those from well differentiated tubular adenocarcinoma.
Cell lines CVCL_0371; KATO III
CVCL_1415; MKN1
CVCL_1417; MKN7
CVCL_1416; MKN28
CVCL_0434; MKN45
CVCL_2791; MKN74
CVCL_9815; Okajima
CVCL_1687; SCH