Cellosaurus - SARS-CoV-2 relevant information
(A) Cell lines that can be used to grow SARS-CoV-2
The information concerning the susceptibility of cell lines to SARS-Cov-2 infection
is now annotated in the relevant Cellosaurus entries
See also the review of Kumar et al (
PubMed=33550034).
(B) Engineered cell lines useful for SARS-CoV-2 research
These are mostly cell lines that are expressing genes (such as ACE2) that are
useful to study the entry of SARS-CoV-2 into cells or cell lines that over-express
some of the SARS-CoV-2 viral proteins.
To help retrieve these cell lines, we have created a specific 'group':
SARS-CoV-2 research cell line
(C) Cell lines shown to be not suitable to grow SARS-CoV-2
This information is now annotated in the relevant Cellosaurus entries:
Click here
(D) Cell lines used in COVID-19 vaccine production
A number of SARS-CoV-2 vaccines make use of cell lines to either grow
an engineered adenovirus vector, an attenuated SARS-CoV-2 virus or a
recombinant spike protein that will directly elicit an immune response.
- HEK293 (CVCL_0045) for Gamaleya Gam-COVID-Vac (Sputnik V)
- HEK293SF-3F6 (CVCL_4V95) for CanSino Biologics Convidecia (Ad5-nCOV)
- K562/GM-CSF (CVCL_A8HT) for Sorento Therapeutics STI-6991 (in development)
- PER.C6 TetR (CVCL_A7ME) for Janssen Ad26.COV2.S
- Sf9 (CVCL_0549) for Novavax NVX-CoV2373
- T-REx-293 (CVCL_D585) for AstraZeneca Vaxzevria (ChAdOx1-SARS-COV-2; AZD1222)
- Vero (CVCL_0059) for Bharat Biotech Covaxin (BBV152) and for Sinovax CoronaVac (PiCoVacc)
For some vaccines in development, we are not sure of the cell line used for their production, however we have some clue that:
- CHO-K1 (CVCL_0214) may be used for the candidate Cuban vaccines Soberana 01, Soberana 02 and Soberana Plus (although no information is available on the precise type of CHO cell line used for these vaccines)
- Sf900+ (CVCL_4U10) may be used for the candidate Sanofi vaccine VAT00002/VAT00008
It must be noted that, as described in a specific
FAQ,
vaccines do
NOT contain cell lines.
For a non-controversial overview of the controversy on the use of human fetal cell lines for COVID-19 vaccine development see
the following University of Nebraska Medical Center
page.
(E) Hybridomas producing mAB against SARS-CoV-2 proteins
These are hybridomas raised against:
1) The spike protein (S) [UniProtKB;
P0DTC2]
To retrieve those annotated in the Cellosaurus:
Click here
2) The nucleoprotein (N) [UniProtKB;
P0DTC9]
To retrieve those annotated in the Cellosaurus:
Click here