Protein Identification Tools - Mass differences due to amino acid substitutions (average mass values)
Example:
To look up the mass difference caused by "E->Q" (a Glutamic acid is replaced by a Glutamine), go to the line of the table labeled "E" and follow this line until you get to the column labeled "Q": -0.98. The substitution "E->Q" decreases the mass of your peptide by 0.98.
You may also access the corresponding table based on monoisotopic mass values for the amino acid masses.
To look up the mass difference caused by "E->Q" (a Glutamic acid is replaced by a Glutamine), go to the line of the table labeled "E" and follow this line until you get to the column labeled "Q": -0.98. The substitution "E->Q" decreases the mass of your peptide by 0.98.
You may also access the corresponding table based on monoisotopic mass values for the amino acid masses.
| A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | - | 32.06 | 44.01 | 58.04 | 76.10 | -14.03 | 66.06 | 42.08 | 57.10 | 42.08 | 60.11 | 43.03 | 26.04 | 57.05 | 85.11 | 16.00 | 30.03 | 28.05 | 115.13 | 92.10 | A |
| C | -32.06 | - | 11.95 | 25.98 | 44.04 | -46.09 | 34.00 | 10.02 | 25.04 | 10.02 | 28.05 | 10.97 | -6.02 | 24.99 | 53.05 | -16.06 | -2.03 | -4.01 | 83.07 | 60.04 | C |
| D | -44.01 | -11.95 | - | 14.03 | 32.09 | -58.04 | 22.05 | -1.93 | 13.09 | -1.93 | 16.10 | -0.98 | -17.97 | 13.04 | 41.10 | -28.01 | -13.98 | -15.96 | 71.12 | 48.09 | D |
| E | -58.04 | -25.98 | -14.03 | - | 18.06 | -72.06 | 8.03 | -15.96 | -0.94 | -15.96 | 2.08 | -15.01 | -32.00 | -0.98 | 27.07 | -42.04 | -28.01 | -29.98 | 57.10 | 34.06 | E |
| F | -76.10 | -44.04 | -32.09 | -18.06 | - | -90.12 | -10.04 | -34.02 | -19.00 | -34.02 | -15.98 | -33.07 | -50.06 | -19.05 | 9.01 | -60.10 | -46.07 | -48.04 | 39.04 | 16.00 | F |
| G | 14.03 | 46.09 | 58.04 | 72.06 | 90.12 | - | 80.09 | 56.11 | 71.12 | 56.11 | 74.14 | 57.05 | 40.06 | 71.08 | 99.14 | 30.03 | 44.05 | 42.08 | 129.16 | 106.12 | G |
| H | -66.06 | -34.00 | -22.05 | -8.03 | 10.04 | -80.09 | - | -23.98 | -8.97 | -23.98 | -5.95 | -23.04 | -40.02 | -9.01 | 19.05 | -50.06 | -36.04 | -38.01 | 49.07 | 26.03 | H |
| I | -42.08 | -10.02 | 1.93 | 15.96 | 34.02 | -56.11 | 23.98 | - | 15.01 | 0.00 | 18.03 | 0.94 | -16.04 | 14.97 | 43.03 | -26.08 | -12.05 | -14.03 | 73.05 | 50.02 | I |
| K | -57.10 | -25.04 | -13.09 | 0.94 | 19.00 | -71.12 | 8.97 | -15.01 | - | -15.01 | 3.02 | -14.07 | -31.06 | -0.04 | 28.01 | -41.10 | -27.07 | -29.04 | 58.04 | 35.00 | K |
| L | -42.08 | -10.02 | 1.93 | 15.96 | 34.02 | -56.11 | 23.98 | 0.00 | 15.01 | - | 18.03 | 0.94 | -16.04 | 14.97 | 43.03 | -26.08 | -12.05 | -14.03 | 73.05 | 50.02 | L |
| M | -60.11 | -28.05 | -16.10 | -2.08 | 15.98 | -74.14 | 5.95 | -18.03 | -3.02 | -18.03 | - | -17.09 | -34.08 | -3.06 | 24.99 | -44.11 | -30.09 | -32.06 | 55.02 | 31.98 | M |
| N | -43.03 | -10.97 | 0.98 | 15.01 | 33.07 | -57.05 | 23.04 | -0.94 | 14.07 | -0.94 | 17.09 | - | -16.99 | 14.03 | 42.08 | -27.03 | -13.00 | -14.97 | 72.11 | 49.07 | N |
| P | -26.04 | 6.02 | 17.97 | 32.00 | 50.06 | -40.06 | 40.02 | 16.04 | 31.06 | 16.04 | 34.08 | 16.99 | - | 31.01 | 59.07 | -10.04 | 3.99 | 2.02 | 89.10 | 66.06 | P |
| Q | -57.05 | -24.99 | -13.04 | 0.98 | 19.05 | -71.08 | 9.01 | -14.97 | 0.04 | -14.97 | 3.06 | -14.03 | -31.01 | - | 28.06 | -41.05 | -27.03 | -29.00 | 58.08 | 35.05 | Q |
| R | -85.11 | -53.05 | -41.10 | -27.07 | -9.01 | -99.14 | -19.05 | -43.03 | -28.01 | -43.03 | -24.99 | -42.08 | -59.07 | -28.06 | - | -69.11 | -55.08 | -57.05 | 30.03 | 6.99 | R |
| S | -16.00 | 16.06 | 28.01 | 42.04 | 60.10 | -30.03 | 50.06 | 26.08 | 41.10 | 26.08 | 44.11 | 27.03 | 10.04 | 41.05 | 69.11 | - | 14.03 | 12.05 | 99.14 | 76.10 | S |
| T | -30.03 | 2.03 | 13.98 | 28.01 | 46.07 | -44.05 | 36.04 | 12.05 | 27.07 | 12.05 | 30.09 | 13.00 | -3.99 | 27.03 | 55.08 | -14.03 | - | -1.97 | 85.11 | 62.07 | T |
| V | -28.05 | 4.01 | 15.96 | 29.98 | 48.04 | -42.08 | 38.01 | 14.03 | 29.04 | 14.03 | 32.06 | 14.97 | -2.02 | 29.00 | 57.05 | -12.05 | 1.97 | - | 87.08 | 64.04 | V |
| W | -115.13 | -83.07 | -71.12 | -57.10 | -39.04 | -129.16 | -49.07 | -73.05 | -58.04 | -73.05 | -55.02 | -72.11 | -89.10 | -58.08 | -30.03 | -99.14 | -85.11 | -87.08 | - | -23.04 | W |
| Y | -92.10 | -60.04 | -48.09 | -34.06 | -16.00 | -106.12 | -26.03 | -50.02 | -35.00 | -50.02 | -31.98 | -49.07 | -66.06 | -35.05 | -6.99 | -76.10 | -62.07 | -64.04 | 23.04 | - | Y |