MALDIPepQuant v3.1

User manual (v1.0)

- How to install MALDIPepQuant
- How to start MALDIPepQuant
- How to run MALDIPepQuant
- Content of the output file

# How to install MALDIPepQuant First of all, be sure to have at least version 1.5 of Java Runtime Environment (JRE) installed on your computer. If not, you can download the latest version from the Sun website. Download latest version of the MALDIPepQuant distribution from here. Unzip the archive anywhere on your computer. This will create a new folder called MALDIPepQuant. Installation is done.
# How to start MALDIPepQuant Double click on the file called MALDIPepQuant_3-1_20080306.jar inside the folder MALDIPepQuant/exe/. Main window of MALDIPepQuant should appear.
# How to run MALDIPepQuant Fill out the fields according to the following informations and click on the Start... button. Note: if you put a small file containing the path to the Excel executable in your home folder on your computer, MALDIPepQuant will be able to automatically open its output file after it has finished. ### Label of heavy peptide This is the label used to indicate the position of the heavy amino acid. ### Error Allowed error on the mass (Dalton). ### Amino acid Heavy aminoacid. ### Shift Weight added to the heavy amino acid. ## Data ### Excel file An Excel export from Phenyx. See export named "Silac data export" in the Phenyx management console. ### Peak Lists folder Folder containg all the MS peak lists in mgf format, as generated by a MALDI-TOF/TOF 4700 or 4800 from Applied Biosystems. Click on the Browse button, select one of the MS files and in the "File name" field replace the Label element by a #. For example if the name of the file was pmf_I14_118760352313.txt it will become pmf_#_118760352313.txt ## Options ### Find echo peaks Select echo peaks you want to search. You can select either -1Da either -2Da, or both. ### Find intermediary peaks Select this option if you think that the heavy amino acids were only partially incorporated.
# Content of the output file Output file will be created in the same folder as the original Excel file (Phenyx export) with the "quanti_" prefix added to the original file name. This file contains a copy of the original sheet and one new sheet per exported Phenyx job. ### Columns details Proteins Protein of current peptide. Peptides Sequence of the peptide. Labeled AA nb Number of labeled amino acids in this peptide. Forms Observed forms for this peptide. Masses Labelof the MS spectrum followed by the masses found (light form/heavy form/eventual intermediary forms). Light Form Peak area found for the light form of the peptide. This column is followed by its eventual -1 and -2Da informations. Heavy Form Peak area found for the heavy form of the peptide. This column is followed by its eventual -1 and -2Da informations. Depending on the parameters, it will also be followed by the columns containing informations about the intermediary forms of the peptide. Sum LF Sum of the peak areas of the light forms of the peptide. Sum HF Sum of the peak areas of the heavy forms of the peptide (including eventual intermediary peaks). LF / HF Ratio of the sum of the light forms divided by he sum of heavy forms.