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UniProtKB/Swiss-Prot variant pages

UniProtKB/Swiss-Prot P60484: Variant p.Ile135Val

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Gene: PTEN
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Variant information Variant position: help 135 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant: help LP/P [Disclaimer] The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance

Residue change: help From Isoleucine (I) to Valine (V) at position 135 (I135V, p.Ile135Val). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties: help Similar physico-chemical property. Both residues are medium size and hydrophobic. The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score: help 3 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page

Variant description: help In CWS1. Any additional useful information about the variant.
Other resources: help Links to websites of interest for the variant.


Sequence information Variant position: help 135 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length: help 403 The length of the canonical sequence.
Location on the sequence: help DDNHVAAIHCKAGKGRTGVM I CAYLLHRGKFLKAQEALDFY The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Residue conservation: help The multiple alignment of the region surrounding the variant against various orthologous sequences.
Human                         DDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFY

                              DDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFY

Mouse                         DDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFY

Rat                           DDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFY

Xenopus laevis                NEN-VAAIHCKAGKGRTGVMICAYLLHRGKFPRAQEALDFY

Caenorhabditis elegans        DDKHVIAVHCKAGKGRTGVMICALLIYINFYPSPRQILDYY

Slime mold                    DSKNIAVIHCKAGKGRTGLMICCWLMYCGMWKNTEDSLRFY

Fission yeast                 QPLLTLVVHCKAGKGRTGTVICSYLVAFGG-LTAKQSLELY

Sequence annotation in neighborhood: help The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.
TypePositionsDescription
Chain 2 – 403 Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Domain 14 – 185 Phosphatase tensin-type
Active site 124 – 124 Phosphocysteine intermediate
Mutagenesis 124 – 124 C -> A. Loss of protein phosphatase activity. Unable to inhibit focal adhesion formation.
Mutagenesis 124 – 124 C -> S. Loss of phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity.
Mutagenesis 125 – 125 K -> M. Reduced phosphatase activity towards PtdIns(3,4,5)P3, PtdIns(3,4)P2 and PtdIns(3)P.
Mutagenesis 126 – 126 A -> P. Does not reduce phosphatase activity towards PtdIns(3,4,5)P3 and PtdIns(3,4)P2.
Mutagenesis 126 – 126 A -> S. Does not reduce phosphatase activity towards PtdIns(3,4,5)P3 and PtdIns(3,4)P2.
Mutagenesis 126 – 126 A -> V. Does not reduce phosphatase activity towards PtdIns(3,4,5)P3 and PtdIns(3,4)P2.
Mutagenesis 128 – 128 K -> M. 85% reduction in phosphatase activity towards PtdIns(3,4,5)P3.
Mutagenesis 128 – 128 K -> R. Does not reduce phosphatase activity towards PtdIns(3,4,5)P3.
Mutagenesis 130 – 130 R -> M. Does not affect the ability to inhibit AKT/PKB activation.
Helix 129 – 141



Literature citations
PTEN mutation spectrum and genotype-phenotype correlations in Bannayan-Riley-Ruvalcaba syndrome suggest a single entity with Cowden syndrome.
Marsh D.J.; Kum J.B.; Lunetta K.L.; Bennett M.J.; Gorlin R.J.; Ahmed S.F.; Bodurtha J.; Crowe C.; Curtis M.A.; Dasouki M.; Dunn T.; Feit H.; Geraghty M.T.; Graham J.M. Jr.; Hodgson S.V.; Hunter A.; Korf B.R.; Manchester D.; Miesfeldt S.; Murday V.A.; Nathanson K.L.; Parisi M.; Pober B.; Romano C.; Tolmie J.L.; Trembath R.; Winter R.M.; Zackai E.H.; Zori R.T.; Weng L.-P.; Dahia P.L.M.; Eng C.;
Hum. Mol. Genet. 8:1461-1472(1999)
Cited for: VARIANTS CWS1 ASP-34; HIS-68; TYR-105; VAL-135; ARG-170 AND LEU-246; Rapid mutation scanning of genes associated with familial cancer syndromes using denaturing high-performance liquid chromatography.
Marsh D.J.; Theodosopoulos G.; Howell V.; Richardson A.-L.; Benn D.E.; Proos A.L.; Eng C.; Robinson B.G.;
Neoplasia 3:236-244(2001)
Cited for: VARIANTS CWS1 ASP-34; GLY-47; HIS-68; TYR-105; VAL-135 AND ARG-170;
Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.