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UniProtKB/Swiss-Prot variant pages

UniProtKB/Swiss-Prot P60484: Variant p.Phe347Leu

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Gene: PTEN
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Variant information Variant position: help 347 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant: help LP/P [Disclaimer] The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance

Residue change: help From Phenylalanine (F) to Leucine (L) at position 347 (F347L, p.Phe347Leu). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties: help Change from large size and aromatic (F) to medium size and hydrophobic (L) The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score: help 0 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page

Variant description: help In CWS1; reduced phosphatase activity towards Ins(1,3,4,5)P4. Any additional useful information about the variant.


Sequence information Variant position: help 347 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length: help 403 The length of the canonical sequence.
Location on the sequence: help KANKDKANRYFSPNFKVKLY F TKTVEEPSNPEASSSTSVTP The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Residue conservation: help The multiple alignment of the region surrounding the variant against various orthologous sequences.
Human                         KANKDKANRY------------------FSPN--------------------------------------------------------------FKVKLY------------------------------FTKTVEEPS----NPEASSS-------TSVTP

                              KANKDKANRY------------------FSPN---------

Mouse                         KANKDKANRY------------------FSPN---------

Rat                           KANKDKANRY------------------FSPN---------

Xenopus laevis                KANKDKANRL------------------FSPN---------

Caenorhabditis elegans        NPEEESCEHKTVESIAGFEPLEHLFHESYHPNTAGNMLRQD

Slime mold                    NNNNNNLNSSNSNNA-------------TTTT---------

Fission yeast                 ----ERKGQF------------------YFPS---------

Sequence annotation in neighborhood: help The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.
TypePositionsDescription
Chain 2 – 403 Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Domain 190 – 350 C2 tensin-type
Region 338 – 348 Required for interaction with NOP53
Modified residue 336 – 336 Phosphotyrosine; by FRK
Modified residue 366 – 366 Phosphothreonine; by GSK3-beta and PLK3
Alternative sequence 191 – 403 Missing. In isoform 3.
Mutagenesis 336 – 336 Y -> F. Significantly lower phosphatase activity, reduced protein stability and decreased growth-inhibitory effect.
Mutagenesis 366 – 366 T -> A. Decreased stability.
Beta strand 342 – 349



Literature citations
Inherited mutations in PTEN that are associated with breast cancer, Cowden disease, and juvenile polyposis.
Lynch E.D.; Ostermeyer E.A.; Lee M.K.; Arena J.F.; Ji H.; Dann J.; Swisshelm K.; Suchard D.; MacLeod P.M.; Kvinnsland S.; Gjertsen B.T.; Heimdal K.; Lubs H.; Moeller P.; King M.-C.;
Am. J. Hum. Genet. 61:1254-1260(1997)
Cited for: VARIANTS CWS1 GLU-343 AND LEU-347; Functional evaluation of PTEN missense mutations using in vitro phosphoinositide phosphatase assay.
Han S.-Y.; Kato H.; Kato S.; Suzuki T.; Shibata H.; Ishii S.; Shiiba K.; Matsuno S.; Kanamaru R.; Ishioka C.;
Cancer Res. 60:3147-3151(2000)
Cited for: CHARACTERIZATION OF VARIANTS ASN-10; CYS-16; GLU-20; SER-27; ARG-61; HIS-68; ARG-112; PRO-121; ARG-129; GLY-130; ILE-133; LEU-134; ARG-165; ASN-170; CYS-173; HIS-173; PRO-173; ASN-174; PHE-227; CYS-251; GLN-345; GLY-369 AND ILE-401; CHARACTERIZATION OF VARIANTS CWS1 TYR-71; TYR-93; PHE-105; TYR-107; PRO-112; ARG-124; GLU-129; LEU-130; GLN-130; TYR-136; CYS-155; ARG-170; GLU-289; GLY-331; VAL-341; ASN-342; GLU-343 AND LEU-347;
Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.