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UniProtKB/Swiss-Prot P18848: Variant p.Pro258Ala

Cyclic AMP-dependent transcription factor ATF-4
Gene: ATF4
Variant information

Variant position:  258
The position of the amino-acid change on the UniProtKB canonical protein sequence.

Type of variant:  LB/B
The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance

Residue change:  From Proline (P) to Alanine (A) at position 258 (P258A, p.Pro258Ala).
Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.

Physico-chemical properties:  Change from medium size and hydrophobic (P) to small size and hydrophobic (A)
The physico-chemical property of the reference and variant residues and the change implicated.

BLOSUM score:  -1
The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page

Other resources:  
Links to websites of interest for the variant.



Sequence information

Variant position:  258
The position of the amino-acid change on the UniProtKB canonical protein sequence.

Protein sequence length:  351
The length of the canonical sequence.

Location on the sequence:   TRGSPNRSLPSPGVLCGSAR  P KPYDPPGEKMVAAKVKGEKL
The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.

Residue conservation: 
The multiple alignment of the region surrounding the variant against various orthologous sequences.

Human                         T-RGSPNRSLPSPGVLCGSARPKPY---DPPGEKMVAAKVKGE----KL

Mouse                         TSRAPPD-NLPSPGGSRGSPRPKPY---DPPGVS-LTAKVK

Rat                           TSRAPPD-SLPSPGVPRGS-RPKPY---DPPGVS-VTAKVK

Bovine                        TSRGSPNKSLLSPGALSGSSRPKPY---DPPGEKMVAAKVK

Zebrafish                     ---------------LEPSSRAKPYSRPDPEASPALKGRVK

Sequence annotation in neighborhood:  
The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.

TypePositionsDescription
Chain 1 – 351 Cyclic AMP-dependent transcription factor ATF-4
Region 210 – 268 Disordered
Modified residue 245 – 245 Phosphoserine; by RPS6KA3
Modified residue 248 – 248 Phosphoserine
Cross 259 – 259 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Cross 267 – 267 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Cross 272 – 272 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Mutagenesis 245 – 245 S -> A. Abolished phosphorylation by RPS6KA3/RSK2.


Literature citations

No reference for the current variant in UniProtKB/Swiss-Prot.

Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.