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UniProtKB/Swiss-Prot variant pages

UniProtKB/Swiss-Prot Q96P20: Variant p.Leu355Pro

NACHT, LRR and PYD domains-containing protein 3
Gene: NLRP3
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Variant information Variant position: help 355 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant: help LP/P [Disclaimer] The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance
Residue change: help From Leucine (L) to Proline (P) at position 355 (L355P, p.Leu355Pro). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties: help Similar physico-chemical property. Both residues are medium size and hydrophobic. The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score: help -3 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page
Variant description: help In FCAS1. Any additional useful information about the variant.
Other resources: help Links to websites of interest for the variant.


Sequence information Variant position: help 355 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length: help 1036 The length of the canonical sequence.
Location on the sequence: help LIRKKLLPEASLLITTRPVA L EKLQHLLDHPRHVEILGFSE The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Residue conservation: help The multiple alignment of the region surrounding the variant against various orthologous sequences. 
Human                         LIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSE

Rhesus macaque                LIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSE

Mouse                         LIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSE

Rat                           LIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSE

Bovine                        LIRKRLLPEASLLITTRPVALEKLQHLLGQARHVEILGFSE
Sequence annotation in neighborhood: help The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.
TypePositionsDescription
Chain 1 – 1036 NACHT, LRR and PYD domains-containing protein 3
Domain 220 – 536 NACHT
Motif 355 – 359 KFERQ-like motif 1
Mutagenesis 351 – 351 R -> T. Abolished binding to small-inhibitor MCC950 and ability to activate the NLRP3 inflammasome following stimulation with nigericin.
Mutagenesis 359 – 359 Q -> AR. Does not affect ability to activate the NLRP3 inflammasome.
Mutagenesis 359 – 359 Q -> A. Decreased interaction with HSPA8/HSC70 and NLRP3 degradation by the chaperone-mediated autophagy pathway.
Mutagenesis 364 – 364 H -> E. Does not affect ability to activate the NLRP3 inflammasome.
Helix 355 – 358



Literature citations
Fine structure mapping of CIAS1: identification of an ancestral haplotype and a common FCAS mutation, L353P.
Hoffman H.M.; Gregory S.G.; Mueller J.L.; Tresierras M.; Broide D.H.; Wanderer A.A.; Kolodner R.D.;
Hum. Genet. 112:209-216(2003)
Cited for: VARIANT FCAS1 PRO-355; VARIANT LYS-705;
Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.