Type | Positions | Description |
Chain |
1 – 89 |
Barrier-to-autointegration factor |
Initiator methionine |
1 – 1 |
Removed; alternate |
Chain |
2 – 89 |
Barrier-to-autointegration factor, N-terminally processed |
Modified residue |
1 – 1 |
N-acetylmethionine |
Modified residue |
2 – 2 |
(Microbial infection) Phosphothreonine; by viral VacV B1 kinase |
Modified residue |
2 – 2 |
N-acetylthreonine; in Barrier-to-autointegration factor, N-terminally processed |
Modified residue |
2 – 2 |
Phosphothreonine; by VRK1 and VRK2 |
Modified residue |
3 – 3 |
(Microbial infection) Phosphothreonine; by viral VacV B1 kinase |
Modified residue |
3 – 3 |
Phosphothreonine; by VRK1 and VRK2 |
Modified residue |
4 – 4 |
Phosphoserine; by viral VacV B1 kinase, VRK1 and VRK2 |
Mutagenesis |
4 – 4 |
S -> A. Delayed phosphorylation with a 10-fold decrease in the initial phosphorylation rate. 71% loss of binding to lamin A. |
Mutagenesis |
4 – 4 |
S -> D. 75% cytoplasmic localization. |
Mutagenesis |
4 – 4 |
S -> E. Complete loss of phosphorylation and mislocalization of EMD in nucleus. |
Mutagenesis |
6 – 6 |
K -> A. Complete loss of LEMD3/MAN1 and histone H1/H3 binding. |
Mutagenesis |
6 – 6 |
K -> E. Complete loss of dsDNA and LEMD3/MAN1 binding. |
Mutagenesis |
8 – 8 |
R -> A. Enhances histone H1/H3 binding. |
Mutagenesis |
8 – 8 |
R -> E. Complete loss of LEMD3/MAN1 binding. |
Mutagenesis |
9 – 9 |
D -> A. Reduces binding to dsDNA, LEMD3/MAN1 and histone H1/H3. Reduced interaction with PARP1. |
Mutagenesis |
14 – 14 |
P -> A. No effect on LEMD3/MAN1 and enhances histone H1/H3 binding. |
Mutagenesis |
18 – 18 |
K -> A. No effect on histone H1/H3 binding. |
Mutagenesis |
25 – 25 |
G -> E. Complete loss of dsDNA, EMD, histone H1/H3 and LEMD3/MAN1 binding. |
Mutagenesis |
25 – 25 |
G -> Q. Complete loss of EMD binding and reduces dsDNA binding. |
Mutagenesis |
26 – 26 |
I -> A. Reduces histone H1/H3 and LEMD3/MAN1 binding. Fails to promote HIV-1 genome integration. |
Mutagenesis |
26 – 26 |
I -> K. Fails to promote HIV-1 genome integration. |
Mutagenesis |
27 – 27 |
G -> E. Fails to bind dsDNA. |
Mutagenesis |
27 – 27 |
G -> Q. Reduces binding to dsDNA. |
Mutagenesis |
29 – 29 |
V -> A. No effect on histone H1/H3 binding. |
Mutagenesis |
32 – 32 |
K -> E. No effect on histone H1/H3 binding. |