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UniProtKB/Swiss-Prot variant pages

UniProtKB/Swiss-Prot Q9UMS0: Variant p.Gly208Cys

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
Gene: NFU1
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Variant information Variant position: help 208 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant: help LP/P [Disclaimer] The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance
Residue change: help From Glycine (G) to Cysteine (C) at position 208 (G208C, p.Gly208Cys). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties: help Change from glycine (G) to medium size and polar (C) The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score: help -3 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page
Variant description: help In MMDS1; patient's skeletal muscles and fibroblasts show deficiency of mitochondrial respiratory chain complexes; increases homodimerization; unable to receive a Fe/S clusters from donor proteins; changes delivery rates of [2Fe-2S] cluster to target proteins. Any additional useful information about the variant.
Other resources: help Links to websites of interest for the variant.


Sequence information Variant position: help 208 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length: help 254 The length of the canonical sequence.
Location on the sequence: help DGGDVIYKGFEDGIVQLKLQ G SCTSCPSSIITLKNGIQNML The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Residue conservation: help The multiple alignment of the region surrounding the variant against various orthologous sequences. 
Human                         DGGDVIYKGFE--DGIVQLKLQGSCTSCPSSIITLKNGIQNML

Mouse                         DGGDVIYRGFE--DGIVRLKLQGSCTSCPSSIITLKSGIQN

Drosophila                    DGGDIVFMGYE--GGVVKLKMQGSCSSCPSSIVTLKNGVQN

Baker's yeast                 DGGDIDYRGWDPKTGTVYLRLQGACTSCSSSEVTLKYGIES
Sequence annotation in neighborhood: help The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.
TypePositionsDescription
Chain 10 – 254 NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
Region 173 – 241 NifU
Binding site 210 – 210
Binding site 213 – 213
Mutagenesis 189 – 189 G -> A. Alters protein structure. Increases likelihood of existing as monomer. Decreases ability to receive a Fe/S clusters from donor proteins. Decreases delivery rates of [2Fe-2S] cluster to target proteins.
Mutagenesis 189 – 189 G -> K. Alters protein structure. Increases likelihood of existing as monomer. Decreases ability to receive a Fe/S clusters from donor proteins. Decreases delivery rates of [2Fe-2S] cluster to target proteins.
Turn 208 – 212



Literature citations
A fatal mitochondrial disease is associated with defective NFU1 function in the maturation of a subset of mitochondrial Fe-S proteins.
Navarro-Sastre A.; Tort F.; Stehling O.; Uzarska M.A.; Arranz J.A.; Del Toro M.; Labayru M.T.; Landa J.; Font A.; Garcia-Villoria J.; Merinero B.; Ugarte M.; Gutierrez-Solana L.G.; Campistol J.; Garcia-Cazorla A.; Vaquerizo J.; Riudor E.; Briones P.; Elpeleg O.; Ribes A.; Lill R.;
Am. J. Hum. Genet. 89:656-667(2011)
Cited for: VARIANT MMDS1 CYS-208; Clinical, biochemical, and genetic spectrum of seven patients with NFU1 deficiency.
Ahting U.; Mayr J.A.; Vanlander A.V.; Hardy S.A.; Santra S.; Makowski C.; Alston C.L.; Zimmermann F.A.; Abela L.; Plecko B.; Rohrbach M.; Spranger S.; Seneca S.; Rolinski B.; Hagendorff A.; Hempel M.; Sperl W.; Meitinger T.; Smet J.; Taylor R.W.; Van Coster R.; Freisinger P.; Prokisch H.; Haack T.B.;
Front. Genet. 6:123-123(2015)
Cited for: VARIANTS MMDS1 PRO-21; TRP-182; ARG-189; ARG-190 AND CYS-208; CHARACTERIZATION OF VARIANTS MMDS1 PRO-21; TRP-182 AND CYS-208; Understanding the Molecular Basis of Multiple Mitochondrial Dysfunctions Syndrome 1 (MMDS1)-Impact of a Disease-Causing Gly208Cys Substitution on Structure and Activity of NFU1 in the Fe/S Cluster Biosynthetic Pathway.
Wachnowsky C.; Wesley N.A.; Fidai I.; Cowan J.A.;
J. Mol. Biol. 429:790-807(2017)
Cited for: CHARACTERIZATION OF VARIANT MMDS1 CYS-208;
Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.