UniProtKB/Swiss-Prot Q96J94: Variant p.Asn224His

Piwi-like protein 1
Gene: PIWIL1
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Variant information Variant position:

224 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant:

US The variants are classified into three categories: LP/P, LB/B and US.

LP/P: likely pathogenic or pathogenic.
LB/B: likely benign or benign.
US: uncertain significance

Residue change:

From Asparagine (N) to Histidine (H) at position 224 (N224H, p.Asn224His). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties:

Similar physico-chemical property. Both residues are medium size and polar. The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score:

1 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:

Lowest score: -4 (low probability of substitution).
Highest score: 11 (high probability of substitution).

More information can be found on the following page
Variant description:

Found in a patient with azoospermia; likely pathogenic. Any additional useful information about the variant.

Sequence information Variant position:

224 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length:

861 The length of the canonical sequence.
Location on the sequence:

TSPTCLQFYNIIFRRLLKIM N LQQIGRNYYNPNDPIDIPSH The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Residue conservation:

The multiple alignment of the region surrounding the variant against various orthologous sequences.

Human                         TSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSH

Mouse                         TSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNH

Chicken                       TSPTCLQFYNIIFRRLLKMMNFQQIGRNYYNPKDPVSIPNH

Zebrafish                     SSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDPFNIPQH

Sequence annotation in neighborhood:

The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:

Type: the type of sequence feature.
Positions: endpoints of the sequence feature.
Description: contains additional information about the feature.

Type	Positions	Description
Chain	1 – 861	Piwi-like protein 1
Motif	217 – 224	D-box

Literature citations
Ubiquitination-deficient mutations in human Piwi cause male infertility by impairing histone-to-protamine exchange during spermiogenesis.
Gou L.T.; Kang J.Y.; Dai P.; Wang X.; Li F.; Zhao S.; Zhang M.; Hua M.M.; Lu Y.; Zhu Y.; Li Z.; Chen H.; Wu L.G.; Li D.; Fu X.D.; Li J.; Shi H.J.; Liu M.F.;
Cell 169:1090-1104(2017)
Cited for: VARIANTS ALA-217; ALA-220; ARG-220; GLY-220 AND HIS-224; INVOLVEMENT IN AZOOSPERMIA; UBIQUITINATION;

Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.