PeptideCutter

PeptideCutter - Peptide Characterisation Software

PeptideCutter [Documentation / References] predicts potential cleavage sites cleaved by proteases or chemicals in a given protein sequence. PeptideCutter returns the query sequence with the possible cleavage sites mapped on it and /or a table of cleavage site positions.

Please enter one UniProtKB AC/ID (e.g. P04406 or ALBU_HUMAN). Alternatively, enter one protein sequence in single letter code (e.g. SERVELAT):
Examples

the cleavage of the protein. the fields.

Please, select clevage enzymes or chemicals
all available enzymes and chemicals
only the following selection of enzymes and chemicals

Arg-C proteinase	Asp-N endopeptidase	Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole	Caspase1	Caspase2
Caspase3	Caspase4	Caspase5
Caspase6	Caspase7	Caspase8
Caspase9	Caspase10
Chymotrypsin-high specificity (C-term to [FYW], not before P)	Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)	CNBr	Enterokinase
Factor Xa	Formic acid	Glutamyl endopeptidase
GranzymeB	Hydroxylamine	Iodosobenzoic acid
LysC	LysN	NTCB (2-nitro-5-thiocyanobenzoic acid)
Neutrophil elastase
Pepsin (pH1.3)	Pepsin (pH>2)	Proline-endopeptidase
Proteinase K	Staphylococcal peptidase I	Tobacco etch virus protease
Thermolysin	Thrombin	Trypsin

for the following enzymes an additional, more sophisticated model can be applied that attributes a probability of cleavage to each site :

Please enter the lowest cleavage probability that you would like to be displayed: %

Please indicate the way you would like the cleavage sites to be displayed
Map of cleavage sites. Please select the number of amino acid within one block:
Table of sites, sorted alphabetically by enzyme and chemical name
Table of sites, sorted sequentially by amino acid number

Please indicate which enzymes to include in the display
All enzymes and chemicals
Enzymes and chemicals cleaving exactly times
Enzymes and chemicals cleaving at least times, and at most times

the cleavage of the protein. the fields.