GlycoMod

GlycoMod is a tool that can predict the possible oligosaccharide structures that occur on proteins from their experimentally determined masses. The program can be used for free or derivatized oligosaccharides and for glycopeptides [Documentation / Mass values / References / Disclaimer].

Note: You can use GlycanMass to calculate the mass of an oligosaccharide structure from its oligosaccharide composition.

Experimental masses:

Enter a list of experimental masses (one mass per line):
Example

Or
Upload a file containing one mass per line:

All mass values are average or monoisotopic.
Mass tolerance: +/-

Ion mode and adducts:

positive	negative	neutral
[M+H]⁺	[M-H]^-
Na⁺ or K⁺	acetate or trifluoroacetic acid	[M]
other: mass:	other: mass:

Glycan form:

N-linked oligosaccharides O-linked oligosaccharides

Form of N-linked oligosaccharide:

Form of O-linked oligosaccharide:

As you selected Glycopeptides, please specify either:

A protein sequence or a UniProtKB AC or ID:: Examples

Enzyme:
max. missed cleavage sites (MC).
Cysteines treated with:
acrylamide adducts on cysteines methionines oxidized
A set of unmodified peptide masses ([M]):: These masses must be average or monoisotopic in agreement with that specified above for experimental masses.
Example

As you selected Derivatized oligosaccharides, please specify:

Reducing terminal derivative name: Mass:

Monosaccharide residues present (if known):

Monosaccharide residues are: underivatized permethylated peracetylated.

		range (if known):
Hexose (e.g. Man, Gal)		-
HexNAc (e.g. GlcNAc, GalNAc)		-
Deoxyhexose (e.g. fucose)		-
NeuAc (e.g. sialic acid)		-
NeuGc		-

		range (if known:)
Pentose (e.g. xylose)		-
Sulphate		-
Phosphate		-
KDN		-
HexA (e.g. glucuronic acid)		-

Output parameter

List compositions reported in GlyConnect separately.